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KMID : 1007520140230041253
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Food Science and Biotechnology
2014 Volume.23 No. 4 p.1253 ~ p.1259
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Anti-osteoclastogenic, Estrogenic, and Antioxidant Activities of Cell Suspension Cultures and Tuber Root Extracts from Pueraria mirifica
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Tanatorn Saisavoey
Tanapat Palaga
Suchinda Malaivijitnond
Sukanya Jaroenporn
Nuttha Thongchul
Aphichart Karnchanatat
Polkit Sangvanich
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Abstract
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The flavonoid contents of intact tubers and cell culture media were determined and physiological activities of Pueraria mirifica extracts were investigated. The total flavonoid contents from cell culture media (PMC) were higher than from tubers (PMT). Results from in vivo estrogenic activity assays indicated that PMT had a strong estrogenic activity in ovariectomized rats. The same amount of PMC exhibited a weak activity. In vitro osteoclast suppression investigations indicated that both PMT and PMC extracts exhibited anti-osteoclastogenic activities with low toxicities in a standard test cell line. Determination of the antioxidant potential using the DPPH assay revealed that the IC50 value for PMT was lower than for PMC. P. mirifica cell cultures produce more flavonoids and exhibit a mild estrogenic and more antioxidant activities than tubers.
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KEYWORD
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Pueraria, anti-osteoclastogenic, estrogen, antioxidant
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